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Figure 4 | BMC Developmental Biology

Figure 4

From: C. elegans serine-threonine kinase KIN-29 modulates TGFβ signaling and regulates body size formation

Figure 4

mRNA levels of the Sma/Mab target gene, lon-1 , are negatively regulated in kin-29(wk61) animals. Northern blot showing lon-1 mRNA expression observed in mixed stage populations of N2, sma-6(wk7), sma-3(wk30), and kin-29(wk61) animals. Sma/Mab pathway mutants sma-6(wk7) and sma-3(wk30) show an up-regulation of the lon-1 transcript (lanes 2 and 3). Similarly, kin-29(wk61) also shows an increase in lon-1 mRNA (lane 4). Elongation factor-2 (eft-2) was used to control for amounts of RNA loaded per lane. Levels of mRNA were quantitated using a phosphorimager and IQMacv1.2 software. See Materials and Methods for details on relative transcript level calculations.

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