Figure 4

Dl promotes expression of fng and pan. A. Northern blots showing fng and pan expression in the indicated cell mixtures at 0 and 45 minutes after cell mixing. B. Northern blots showing fng and pan expression in the indicated cell lines two hours after induction of expression. C. Northern blots showing fng and pan expression in two other independently established S2-Dl cell lines. Cells used for lanes 1–2 were uninduced (ui); cells used for lanes 3–4 were heat shock induced. D. Northern blots showing fng and pan expression in S2-Dl cells that were either untreated or treated with Sca medium. All experiments were repeated at least three times. The fng band marked with an asterisk corresponds to the published mRNA [35]. Only this band was used for fng quantification. The pan band shown is consistent with the information described in van de Wetering et al. [40] and Brunner et al. [39].