The sequence of the sea urchin major vault protein (SpMVP). (A) Amino acid sequence of SpMVP aligned with that of rat MVP. Identical residues are highlighted in yellow, and similar residues are highlighted in green. (B) Bar diagram of SpMVP. The sequence has seven repeats in the N-terminal half of the protein (R1-R7), each repeat consisting of 41 to 62 aa, spanning residues 32–400; and a predicted coiled coil region in the C-terminal half of the sequence (C.C.), residues 663–762. The positions of two possible nuclear localization sequences (NLS1 470-KKAR, and NLS2 498-KPKR), a putative nuclear export sequence (NES, 792–816), and two probable sumoylation sites that are conserved across six species (S1 K308-VKGE, and S2 K707-AKAE) are indicated.