Figure 4

Clones of cells expressing HhN, which lacks the cholesterol moiety, generate moderate Hh responses, over an increased range. A, e, i and m: clone marker (LacZ); b and f: Ci; c and g: En, j and n: Col; k and o: Hh. D, h, l and p: merged images across rows (d and h: clone marker in green, Ci in red, En in blue; l and p: clone marker in blue, Col in red, Hh in green). a–d Ci is up-regulated ubiquitously in disc containing HhN expressing clones, so that the normal strip of elevated expression near the A/P boundary is lost. The entire anterior part of the disc is overgrown (compare with fig 1a–d). e–h. Higher magnification of clones. There is no local effect on Ci (no change within target groups 0–2) although Ci expression is high generally. En is weakly up-regulated in most of the wing pouch. There is very little up-regulation confined to the target group cells. i–l Col is up-regulated in and around clones but expression also extends in two broad stripes through much of the pouch. It can also be seen that the clone cells do express Hh. m–p. Higher magnification images of clones like those shown in i–l. It can be seen that Col is up-regulated in and around the clone (target groups 0, 1 and 2) but is also up-regulated in broad domains extending away from clones. These domains encompass much of the anterior wing pouch but exclude the wing margin.