Figure 2

Clones of cells expressing full-length Hh recapitulate the full range of Hh responses. a, e, i, m and q: lacZ, clone marker; b, f, j: Ci; c, g and k: En; n and r: Col; o and s: Hh. D, h, l, p and t: merged images across rows (d, h and l: clone markers in green; En in blue and Ci in red. P and t: clone marker in blue, Col in red and Hh in green). a–d, Ci (red) is down-regulated and En (blue) is up-regulated in and around clones expressing Hh-FL. The anterior pouch of these discs is overgrown (a–d). e–h. Higher magnification of clone similar to those in a–d, showing target cell groups 0 (cells within clone), 1 (2–3 cell diameters immediately outside clone) and 2 (cells further from clone), (see text for details). i–l It can be seen clearly that Ci is down-regulated within and around the clone (in target groups 0 and 1) but is up-regulated further from the clone (target group 2), whereas En is up-regulated in and immediately around the clone (in target groups 0 and 1, but not 2). m–p. Collier is up-regulated in and around clones. It can also be seen that these cells express Hh (green). q–t. Higher magnification images of clones like those shown in m–p. It can be seen that Col is up-regulated in and around the clone (target groups 0, 1 and, at a lower level, 2)