Chronic inflammation following in utero AdCMVAScftr therapy. Lung fields from rats at 100 days were examined for inflammation following hematoxylin and eosin staining (Panels A & B). Prominent areas of inflammatory cell infiltrate surrounding membranous airways were demonstrated in AdCMVAScftr treated rats (Panel B) and were not found in AdCMVgfp control animals (Panel A). Staining of the areas of inflammatory cell infiltrate with Masson's Trichrome demonstrated interstitial fibrosis associated with the inflammation (Panels C and D). Original magnification Panels A, B and C-100×; Panel D-400×.