Figure 4

Effect of Glce knockdown on embryo morphogenesis. (a-d) Embryos at 1–2 cells stage were injected with a mix (8 ng each) of glce-A and glceB MO and the phenotype scored at 48 hpf. (a) wild type embryo. (b,c) mild phenotypes displaying reduced head volume and ventral fin extension. (d) severe phenotype with shortened A/P axis and loss of ventral structures. (e) Epimerase enzymatic activity in 10 hpf embryos and effect of Glce knockdown. The enzymatic assay was performed as described in Fig. 2b. (f-p) Whole mount in-situ hybridization with D/V markers of embryos at different developmental stages. (f) gata1 expression in wild-type embryos, (g) glce morphants, and (h) in embryos overexpressing glce at 24 hpf. (i-l) chordino at 50% epiboly in wild type (top) and morphants (bottom). (i,j) animal pole view, (k,l) dorsal view. (m,n) bmp2b at 70% epiboly and (o,p) at 3 somite stage in wild type (top) and morphants (bottom). Note the absence of bmp2b expression in the presumptive tail of the morphants as indicated by the arrows.