Table 1 Comparison of the specificity for monosaccharides and oligosaccharides of a panel of 14 biotinylated lectins used in immunocytochemistry and flow cytometry.
From: Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces
Lectin Origin | Monosaccharide Specificity | Inhibitor |
|---|---|---|
Concanavalin A (Con A) | Man or Glc | 200 mM α-methylmannoside or α-methyl glucoside |
Phaseolus vulgaris erythro-agglutinin (PHA-E) | Gal | galactose |
Phaseolus vulgaris leuco-agglutinin (PHA-L) | Gal | galactose |
Sambucus nigra agglutinin (SNA) | Sialic Acid | 500 mM lactose in acetic acid |
Arachis hypogea peanut (PNA) | Gal | 200 mM galactose |
Vicia villosa agglutinin (VVA) | GalNAc | 200 mM N-acetylgalactosamine |
Maackia amurensis (MAA) | Gal | 200 mM lactose |
Ricinus communis agglutinin (RCA) | Gal | 200 mM galactose or lactose |
Wisteria floribunda agglutinin (WFA) | GalNAc | 200 mM N-acetylgalactosamine |
Ulex europaeus agglutinin (UEA) | Fuc | 50–100 mM L-fucose |
Lotus tetragonolobus lectin (LTL) | Fuc | 50–100 mM L-fucose |
Dolichos biflorus agglutinin (DBA) | GalNAc | 200 mM N-acetylgalactosamine |
Hippeastrum hybrid lectin (HHL) | Man | 100 mM mannose |
Lycopersicon esculetum tomato (TL) | GlcNAc | Chitin Hydrolysate |