Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Table 1 Combinations of medium supplements used at different culture stages

Supplement combination	Day 3–5 (hanging drops)	Day 5 onwards (attached culture)
A	BMP-2
B	TGFβ₁
C	BMP-2 TGFβ₁
D	BMP-2 TGFβ₁ insulin ascorbic acid
E	BMP-2	insulin ascorbic acid
F	BMP-2 TGFβ₁	insulin ascorbic acid
G	BMP-2 TGFβ₁	insulin ascorbic acid BMP-2
H	TGFβ₁	insulin ascorbic acid

Applied concentrations were 10 ng/ml BMP-2, 2 ng/v ml TGFβ₁, 1 μg/ml insulin and 50 μg/ml ascorbic acid. The end of the culture period varied with every experiment carried out.

ISSN: 1471-213X