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Table 1 Combinations of medium supplements used at different culture stages

From: Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Supplement combination Day 3–5 (hanging drops) Day 5 onwards (attached culture)
A BMP-2  
B TGFβ1  
C BMP-2
TGFβ1
 
D BMP-2
TGFβ1
insulin
ascorbic acid
 
E BMP-2 insulin
ascorbic acid
F BMP-2
TGFβ1
insulin
ascorbic acid
G BMP-2
TGFβ1
insulin
ascorbic acid
BMP-2
H TGFβ1 insulin
ascorbic acid
  1. Applied concentrations were 10 ng/ml BMP-2, 2 ng/v ml TGFβ1, 1 μg/ml insulin and 50 μg/ml ascorbic acid. The end of the culture period varied with every experiment carried out.