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Figure 7 | BMC Developmental Biology

Figure 7

From: Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Figure 7

Characterization of ES-derived adipocytes. (A) Oil-Red-O staining of lipid droplets in ES-derived adipocytes. Bar = 42 μm. (B, C) Influence of BMP-2 alone and in combination with TGFβ1, insulin and ascorbic acid (see table 1) on expression of adipocyte-specific genes. Quantitative RT-PCR was performed on 30 day old EBs. Expression of genes of interest was normalized to GAPDH and compared to untreated controls. Values show means ± standard deviations on n = 3 independent experiments. *P < 0.1; **P < 0.01; ***P = 0.001.

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