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Figure 4 | BMC Developmental Biology

Figure 4

From: Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Figure 4

Expression of cartilage-specific genes in the course of EB differentiation induced by BMP-2, TGFβ1, insulin and ascorbic acid (supplement combination G, table 1). (A) Aggrecan, collagen type II A and B and link protein, biglycan and decorin. (B) Scleraxis and Sox9. Results show induction factors of expression obtained by quantitative RT-PCR and normalized to GAPDH expression in comparison to corresponding spontaneously differentiated controls, which were set as 1 (mean ± standard deviation, n = 3 independent experiments, 20 EBs each).

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