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Figure 2 | BMC Developmental Biology

Figure 2

From: Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Figure 2

Changes on cartilage markers in response to various combinations of BMP-2, TGFβ1, insulin and ascorbic acid as outlined in table 1. (A) Diagram of expression niveaus of cartilage-specific genes of 32 day old EBs obtained by quantitative RT-PCR and standardized to GAPDH. Untreated control values were set as 1. Mean ± standard deviation, n = 3. *P < 0.1; **P < 0.01; ***P = 0.001. (B) Proteoglycan content of extracts of 32 day old EBs treated with different combinations of BMP-2, TGFβ1, insulin and ascorbic acid (see table 1). Ctrl = control. Means ± standard deviation, n = 3. *P < 0.1; **P < 0.01; ***P = 0.001. n.d. = not determined.

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