Figure 8

Preferential inhibition of slow myogenesis by anti-Hh antibody. Schematic drawings of 120 μm spaced sections from each of the seven affected anti-Hh implanted limbs and aligned contralateral controls. Black outline shows the DMM, red stipple indicates slow MyHC. Drawings were made from identical low magnification images of adjacent sections reacted immunohistochemically for A4.1025 or Na8. Two young limbs (HH27/28) show marked reduction in slow MyHC, but little effect on DMM area. Slightly older limbs show a reduction in slow MyHC accompanying a diminished muscle mass. In two limbs, the Cellagen implant is present within the elbow region. Distal limb at top of stack, elbow at base. Within each section dorsal is up and anterior to left. Contralateral limbs are flipped horizontally to aid comparison.