Figure 3

Abnormal pre-implantation development of embryos derived from Dppa3 –deficient oocytes. A,C, Cultured 2-cell (A) and 4-cell (C) control embryos derived from wild-type matings. B,D, Cultured 2-cell (B) and 4-cell (D) embryos produced by crossing Dppa3^tm1WHT/Dppa3^tm1WHTfemales with wild-type males. E,F, E3.5 control embryos derived from wild-type matings have progressed to the blastocyst stage (E). By contrast, most E3.5 embryos produced by crossing Dppa3^tm1WHT/Dppa3^tm1WHTfemales with wild-type males have not progressed to the blastocyst stage and instead cleave abnormally and degenerate (F). G,H, Many embryos produced by crossing Dppa3^tm1WHT/Dppa3^tm1WHTfemales with Dppa3 +/+, Tg^{(Pou5f1 ΔPE-GFP)10WHT}/Tg^{(Pou5f1 ΔPE-GFP)10WHT}males fail to develop normally beyond the 4-cell stage (G) but nonetheless express the Oct4-GFP marker (H).