Figure 6

Analysis of Cut protein expression in the presence of spen mutant clones. Crosses were performed as described in Figure 1, and third instar imaginal disks were isolated from progeny female larvae with the following genotypes: {MS1096 GAL4/+; 40 2piM FRT^40A/ GFP FRT^40A; UAS-Flp1/ +} (A-C), and {MS1096 GAL4/ +; spen^poc361 FRT^40A/ GFP FRT^40A; UAS-Flp1/ +} (D-L). Cut expression (in red) was detected with an anti-Cut MAb as described in Figure 4 for Dl, and the absence of GFP (in green) defines mutant cells as explained on Figure 4. G to H shows a magnification of the spot indicated by an arrow on E and F. Note that the Cut protein is present in spen mutant cells. Panels J to L show the margin of another disc not shown in the figure. The arrow indicates a group of heterozygous cells that are away from the margin, surrounded by a group on spen mutant cells. Dorsal is up and anterior is to the left.