Figure 4

Incorrect positioning of wing elements in mosaic spen mutant wings is enhanced by mutations in the crinkled ( ck ) gene. Virgin females with the genotype {MS1096 GAL4; ck FRT^40A/ ln (2LR) CyO }, or {MS1096 GAL4; spen^poc361ck FRT^40A/ ln (2LR) CyO }, were crossed to {w*; GFP FRT^40A; UAS-Flp1/ TM6b} males, and adult wings were isolated from progeny females with the following genotypes: {MS1096 GAL4/+; ck FRT^40A/ GFP FRT^40A; UAS-Flp1/ +} (A, F), {MS1096 GAL4/+; spen^poc361 ck FRT^40A/ GFP FRT^40A; TM6b/ +} (B-E, G). Red lines separate either wt or compound heterozygous cells (indicated as wt) from spen mutant cells.