Figure 10

Human spen -related genes. The figure shows all Drosophila and human related sequences putatively encoding polypeptides with three RNP-type RNA binding motifs at the N terminus (purple boxes), and the SPOC domain at the C terminus (yellow boxes). The gray box on Hs SHARP indicates the region that contains motifs required for the interaction of SHARP/MINT with Msx-2 (residues 2070 to 2394 [14]), nuclear receptors (residues 2201 to 2707 [15]), and RBP-Jκ (residues 2803 to 2817 [17], and 2638 to 2777 [18]). The expected sizes for each peptide is shown on the right, while names and chromosomal localization in humans is on the left. Asterisks indicate that the peptides have been predicted from the genomic sequence, either because there are no known full length ESTs corresponding to the genomic regions analyzed (case for Hs SSLP at 3p21), or because there are no reported ESTs at all (Hs SSLP at 5q23). In the case of the SSLP at 5q23, there are also stop codons in frame with the putative ORF, so it is likely that this sequence represents a pseudogene. Accession numbers for the sequences likely to represent full length cDNAs are: AAF13218 (Dm SPEN), NP_055816 (Hs SHARP), AAF59160 (Dm SSLP), NP_073605 (OTT/RBM15), and CAC38829 (OTT-MAL fusion). The putative full length ORF for the Hs SSLP at 3p21 was predicted using GENESCAN [54] on the genomic sequence AC092037. The truncated cDNA arising from this gene is found under AAA72367 or NP_037418. The Hs SSLP at 5q23 was predicted with GENESCAN from the genomic segment AC005915, and the assembly was completed with ENSP322787, a predicted peptide from the Ensembl Database [42].