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Figure 1 | BMC Developmental Biology

Figure 1

From: The product of the split ends gene is required for the maintenance of positional information during Drosophiladevelopment

Figure 1

Generation of spen mutant clones in wing imaginal discs. (A) Expression of a UAS-GFP transgene driven by MS1096 GAL4 in third instar wing imaginal discs. As described previously [22], the transgene is expressed mainly in the dorsal wing pouch, with weaker expression in the ventral side and in the prospective notum. (B, C) Virgin females with the genotype {MS1096 GAL4; 40 2piM FRT40A}, or {MS1096 GAL4; spenpoc361 FRT40A/ ln (2LR) CyO}, were crossed to {w*; GFP FRT40A; UAS-Flp1/TM6b} males. Shown are wing imaginal discs isolated from the resulting third instar larvae with the genotypes {MS1096 GAL4/+; 40 2 piM FRT40A/GFP FRT40A; UAS Flp1/ +} (B), and {MS1096 GAL4/+; spenpoc361 FRT40A/GFP FRT40A; UAS-Flp1/ +}(C). Green fluorescence reveals either compound heterozygous cells (not subjected to mitotic recombination) or GFP homozygotes (brightest), while dark spots indicate 2 piM (B) or spenpoc361 (C) homozygous clones. Dorsal is up and anterior is to the left. To know the relative area covered by either 2 piM or spenpoc361 homozygous clones versus wt clones, regions of equal intensity within the images were artificially colored in Adobe Photoshop using the Paint Bucket tool (D, E). Colors corresponding to mutant (red) or wt (blue) areas were extruded independently, and the total number of pixels contained within the regions of interest were calculated using the Kodak 1D Image Analysis Software (Eastman Kodak Company, Rochester, NY). The results are represented as the fraction covered by each genotype for each cross in a total of three discs for the crosses involving 2 piM, and 5 discs for spenpoc361 (F).

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