Regulation of genes with DAF-16 binding sites. In all panels, the bars show groups defined by the location of DAF-16 sites, as shown on the X-axis. We used overlapping intervals to allow robust statistical analysis. Negative numbers are bp upstream of the initiation codon, and positive numbers are bp downstream of the stop codon. Asterisks indicate statistical significance (exact hypergeometric probability) of the proportion of strongly regulated genes in the group compared to all genes:*** is p < 0.001, ** is p < 0.01, * is p < 0.05. A. Strongly upregulated (>2.1 fold, p < 0.01) genes. B. Two fold upregulated genes, excluding those genes in panel A. C. Strongly upregulated genes with downstream DAF-16 sites. D. Strongly downregulated (<2.1 fold, p < 0.01) genes.