Figure 3
RT-PCR for E-ras/RASP, FGFR1 and novel genes identified as enriched in undifferentiated ES cells is shown in Panel A and B. Localization of E-cadherin and β-catenin in undifferentiated ES cell is shown in Panel C. All of the genes identified by MPSS and tested were present in undifferentiated ES cells and most were significantly downregulated as cells differentiated. Note the high expression at the cell surface and low or undetectable levels of β-catenin in the nucleus.