Figure 4

Hsp23 becomes restricted to midline glia by the end of embryogenesis. (A) Localization of Hsp23 in dorsal cells of the ventral nerve cord during late embryogenesis. (B) Detection of hsp23 transcripts in the same subset of cells. (C) Confocal high magnification view of Hsp23 expression in three abdominal segments. Declining Hsp23 expression can still be detected at lower levels in the ventral VUMs. Note the characteristic morphology of the dorsal midline glia (brackets). (D) Confocal high magnification of a similar region shown by brackets in C displaying co-localization of the midline glia marker slit-lacZ (green) and Hsp23 (red). Lateral views of stage 16 embryos are shown in all panels, anterior is towards the left.