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Figure 3 | BMC Developmental Biology

Figure 3

From: Expression of the Hsp23 chaperone during Drosophila embryogenesis: association to distinct neural and glial lineages

Figure 3

Onset of Hsp23 expression in the midline at stage 13. (A) Localization of Hsp23 in a distinct group of cells located ventrally in the nerve cord (arrow). (B) Similar distribution observed by in situ hybridization with hsp23 mRNA. (C) Co-expression of hsp23 mRNA (purple) and Engrailed protein in the novel ventral population of cells. (C') High magnification of region shown by brackets in C. (D-J) Confocal views. (D-E) Co-localization of Hsp23 (red) with Elav (D, green) and sim-lacZ (E, green), suggesting that these cells are neurons of the midline. (F, G) Sequential confocal sections. (F) Hsp23 expression in the two MP2 daughter cells and the ventral group of midline neurons. Note the detection of Hsp23 in the dorsal axonal projection of the vMP2 and dMP2. (G) Presence of Hsp23 in the ventral VUMs neurons and their dorso-anterior projection as well as in the posterior midline glia (MGP) that exhibits a distinctive ventral projection. (H-I) Sequential confocal sections showing the distribution of the X55 enhancer-trap (green) with regards to Hsp23 expression (red). (H) Co-localization in the midline VUMs. (I) Co-localization of X55 and Hsp23 in the VUMs but exclusion of X55 from the MP2 daughter cells located on each side of the midline. (J) Co-localization of Hsp23 and X55 in the MGP. Note that multiple posterior cells expressing X55 (presumptively identified as the MNB and its support cells) do not express Hsp23. Stage 13 embryos are shown in all panels. Ventral views are shown in panels C-E, H-J and lateral views in A, B, F, G. Anterior is towards the left in all panels.

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