Figure 2

The amnioserosa and cells of a single chordotonal organ express Hsp23. (A) Hsp23 expression in cells of the amnioserosa (arrow) and in lateral cells (out of focus – see Fig. 1A) of a stage 11 embryo. (B) Similar distribution shown by hsp23 mRNA transcripts at stage 11. Inset shows hsp23 mRNA detected in two adjacent cells of the lateral region, reminiscent of a mitotic division. (C) Confocal view of a stage 13 embryo displaying Hsp23 expression (red) in cells of a single chordotonal organ per abdominal segments (3 segments indicated by brackets) and in a single dorsal chordotonal organ in segments T2 (white arrow) and T3. Neurons of the peripheral nervous system are identified using the 22C10 molecular marker (green) (D) Confocal high magnification of bracket region in C displaying co-expression of Hsp23 (red) in neuronal cells of lateral chordotonal organs (green). Lateral view is shown in all panels.