Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegansembryonic polarity

Table 1 Polarity and osmotic defects of emb-8 and fatty acid biosynthesis mutants

genotype	symmetric first cleavage (n)^a	osmotically sensitive embryos^b
emb-8(+)	0% (21)	0%
emb-8(hc69)	50% (22)	100%
emb-8(t1462)	0% (16)	100%
emb-8(RNAi)	72% (18)	100%
pod-2(ye60) ^c	43% (28)	100%
pod-2(RNAi)	70% (30)	100%
FAS(RNAi) ^d	89% (19)	100%
HMG-CoA reductase(RNAi) ^e	0% (14)	100%

^a determined by comparison of cross sectional areas as in Rappleye, et al., 1999. ^b osmotic sensitivity determined by absorption of Nile Blue dye by embryos (Rappleye, et al., 2002) or by swelling in hypotonic media. n>50 embryos in all cases. ^c data from Tagawa, et al., 2001 for comparison. ^d open reading frame F32H2.5. ^e open reading frame F08F8.2

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