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Figure 2 | BMC Developmental Biology

Figure 2

From: Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegansembryonic polarity

Figure 2

The emb-8 locus is encoded by K10D2.6, the C. elegans NADPH-cytochrome P450 reductase homolog. (A) Schematic representation of mapping results which place emb-8 in the dpy-17 – unc-32 interval on chromosome III. Sequencing of emb-8 alleles hc69 and t1462 revealed two missense mutations in the predicted gene K10D2.6 which encodes the C. elegans NADPH-cytochrome P450 reductase. Sequencing of two independent clones from wild-type cDNA libraries confirmed the predicted gene structure. RNA-interference of K10D2.6 phenocopies the emb-8(hc69) mutant (B-D). RNAi of the K10D2.6 sequence causes embryos to (B) divide symmetrically at the first division, 41/59; and mislocalization of PAR-2 to (C) reduced posterior domains, 10/31 or (D) posterior-lateral regions of the cortex 14/31. (E) Results of time course experiments for K10D2.6 RNAi using synchronized mid-L4 staged hermaphrodites. Bars represent average proportion of embryos dividing symmetrically from 2–4 experiments with 8 hermaphrodites per experiment per time point (on average 10–15 embryos per time point).

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