Examples of developmental defects in embryos maintained at 16°C at day 42. (A) This embryo has red eyes and an egg burster, both characteristic of embryos about to hatch, but the yolk has not been enclosed through dorsal closure. (B) This embryo has red eyes, but is curled at the posterior of the egg, typical of early katatrepsis (see figure 2H). Embryos are orientated as they would be in the egg, with the anterior of the egg to the left. Scale bars are 100 μm long.