Expression of dFOXO in first instar larvae phenocopiesstarvation and effects feeding behavior. Expression of dFOXOand mFOXO1-AA early in larval development using the (A) ActGal4 and(C) hsGal4 driver lines leads to developmental arrest similarto that seen in starved larvae. Developmentally arrested larvaeare capable of surviving for up to seven days after egg laying (AEL).(B) Expression of dFOXO (red bars) and mFOXO1-AA (green bars) leadsto alterations in feeding behavior when compared to controls (greybars). The percentage of wandering larvae is significantly greaterin larvae expressing dFOXO and mFOXO1-AA at 48 hours and 72 hoursAEL (p = 0.05). Expression of dPI3K-DN (blue bars) did not increaselarval wandering. (D) Developmental arrest is reversible upon removalof dFOXO expression (red bars), but not upon removal of mFOXO1-AAexpression (green bars). Grey bars represent the controls. Eachbar reflects the average of three separate trials, with 50 larvaeper trial. Genotypes are; (A-top, B-grey bars) w; ActGal4/+,(A-middle, B-red bars) w; ActGal4/+; UAS-dFOXO/+, (A-bottom,B-green bars) w, UAS-mFoxo1-AA/w; ActGal4/+, (C-top, D-greybars) w; hsGal4/+, (C-middle, D-red bars), w; hsGal4/UAS-dFOXO,(C-bottom, D-green bars) w, UAS-mFoxo1-AA/w; hsGal4/+,(B-blue bars) w; ActGal4/UAS-dPI3K-DN.