Figure 5

Primary mouse rib chondrocytes in high density bulk cultures. Panel A: Representative culture at 14 days stained with Alcian Blue (100 × magnification); Panel B: Cartilage nodule (200 ×); Panel C: Hypertrophic cells (200 ×); Panel D: Growth curve for primary rib chondrocytes in high-density bulk culture. The linear phase of growth occurs between 48 and 96 hours of plating. From the slope of the curve a mean doubling time of 17 hours (distance between the two green or the two purple lines, respectively) during the linear phase can be calculated.