Skip to main content
Figure 9 | BMC Developmental Biology

Figure 9

From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size

Figure 9

A) Schematic drawing of the 5' leader region of countin and yeast GCN4 mRNAs. The position and size of uORFs are drawn to scale. The first and last uORFs are marked as black boxes, other uORFs are designated by striped boxes, and the open box at the 3' end represents the beginning of the coding region for countin and GCN4. B) Countin and H7 mRNA distributions within the polysomes and ribosome fractions prepared as described in figure 7from BS153 and the parental strain, Ax4, after 4 hours of development. RNA was extracted from the isolated sucrose gradient fractions and was used in RT-PCR reactions with countin or H7 specific oligonucleotides. The numbers refer to the fraction used, and correspond to the numbers given in figure 7. Fractions 3 through 11 represent polysomes with decreasing numbers of associated ribosomes in going from fraction 3 to fraction 11, while fractions 13 through 16 represent free ribosomes. For the countin panels, the BS153 panel was exposed for a longer period of time than that of Ax4 due to the lower amounts of countin mRNA in this strain at four hours post starvation. C) Quantitation of the data shown in panel B for countin mRNA. Within each strain, the relative abundance of countin mRNA from each fraction was determined by quantitating the band in each fraction and dividing this value by the sum of the values of all the fractions for that strain. Black circles, BS153 from 4 hour polysome profile shown in panel B; red circles, BS153 from 0 hour polysome profile; black squares, Ax4 from 4 hour polysome profile shown in panel B; red squares, Ax4 from 0 hour polysome profile.

Back to article page