Figure 7
The polysome profiles in BS153 and the parental strain, Ax4. Lysates, cleared of debris and nuclei, were prepared from both strains 4 hours after the onset of development and were used as a source of polysomes/ribosomes. RNA concentrations in the lysates were determined spectrophotometrically. Sucrose gradient centrifugation was carried out on equal amounts of each sample to generate the polysome profile. The OD at 254 nm was read continuously, and fractions were collected. The black, thin line represents the profile from BS153 and the thicker, gray line is the Ax4 profile. Fractions 3 through 11 represent polysomes with decreasing numbers of associated ribosomes going from fraction 3 to fraction 11, while fractions 13 through 16 represent free ribosomes.