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Figure 6 | BMC Developmental Biology

Figure 6

From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size

Figure 6

eIF2α phosphorylation levels in BS153 and the parental strain, Ax4. Total protein was isolated from growing cells (0) and at the times indicated (in hours) after the onset of development. Equal amounts of each protein sample were separated by PAGE, blotted, and probed with antiserum specific for phosphorylated eIF2α. Stripping of the filter and reprobing with antiserum specific for the V4 protein, a protein found at constant levels in growing and developing cells, indicated equivalent amounts of protein in each lane (not shown).

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