Figure 4

The morphology during development of the ifkA disrupted strain, BS153, and the parental strain, Ax4. Cells of both strains were plated for development under standard conditions. Pictures were taken at the indicated times in hours after the onset of development, all at the same maginification. 7 hr: BS153, tight mounds; Ax4, ripples. 14 hr: BS153 and Ax4, transient slugs; 24 hr: BS153 and Ax4, fruiting bodies. For BS153, several toppled fruiting bodies are indicated by the arrows. Both strains also were developed at a cell density about 6 fold lower than the normal cell density (low cell density panels).