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Figure 3 | BMC Developmental Biology

Figure 3

From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size

Figure 3

Southern analysis of transformants potentially disrupted for ifkA. DNA was isolated from the parental strain, Ax4 (lane 1), and from 4 independently isolated transformants (lanes 2–5) for which PCR had indicated possession of a disrupted ifkA gene. DNA from each was digested with SwaI, subjected to Southern blotting, and probed with a radioactive ifkA fragment. Disruption of the ifkA gene should result in a shift of about 1000 bp due to the insertion of the blasticidin cassette (1400 bp) and the loss of about 400 bp of the endogenous kinase domain. All of the analyzed transformants showed the expected shift, and the strain represented in lane 3 was designated BS153.

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