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Figure 10 | BMC Developmental Biology

Figure 10

From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size

Figure 10

Relative countin mRNA stability in BS153 verses the parental strain, Ax4. Cells from both strains were plated under standard conditions of development after removal of bacteria. After two hours, the filters supporting the cells were transferred to paper pads soaking in fresh PDF plus actinomycin D (actD) or fresh PDF plus actinomycin D and cycloheximide (actD + cyc). After the transfers, a filter of cells was harvested at 0.5, 1, or 2 hours. Total RNA was isolated from these samples and from cells at the time of transfer (0). RT-PCR was carried out using oligonucleotides specific for countin mRNA (upper band in BS153 panel). For the BS153 panel, the band corresponding to H7 mRNA (lower band) is shown as an internal control. The H7 band increases with time in the actD + cyc lanes as H7 transcription is induced by cycloheximide [53].

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