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Table 2 Primers used in quantitative RT-PCR.

From: Stromelysin-1 and mesothelin are differentially regulated by Wnt-5a and Wnt-1 in C57mg mouse mammary epithelial cells

HPRT: forward primer 5'-TGGAAAGAATGTCTTGATTGTTGAA-3'
  reverse primer 5'-AGCTTGCAACCTTAACCATTTTG-3'
BMP-4: forward primer 5'-GCTTGAGTACCCGGAGCGT-3'
  reverse primer 5'-TGTTCTTCGTGATGGAAACTCCT-3'
Plasminogen activator inhibitor-1: forward primer 5'-GGCCTCCAAAGACCGGA-3'
  reverse primer 5'-CAGCATAGCCAGCACCGA-3'
Osteoblast specific factor 2: forward primer 5'-AAGGAAAAGGGTCATACACGTACTTC-3'
  reverse primer 5'-TGCGAATGTCAGAATCCAGGT-3'
Flt4 ligand: forward primer 5'-CAGCTGCGGAAAGGCG-3'
  reverse primer 5'-TTTACACTGTCCCCTGTCCTGG-3'
HTK ligand: forward primer 5'-GCAGGAGACACCGCAAACA-3'
  reverse primer 5'-GTGGCCAGTGTGCTGAGAGA-3'
Mesothelin: forward primer 5'-GCAGTCAGGGAGGTTCTGAGG-3'
  reverse primer 5'-GGTGGAGACTGACCACTTCGA-3'
Stromelysin-1: forward primer 5'-TGGCCATCTCTTCCATCCAA-3'
  reverse primer 5'-CCCAGAACTGATTTCCTTTAAAAATG-3'
Groucho related protein 1 forward primer 5'-CCCTATGCCCCTCATTGGA-3'
  reverse primer 5'-CCGTGGTTGAGGGTGTTGAT-3'
Retinoic acid receptor-alpha: forward primer 5'-AGCACCAGCTTCCAGTCAGTG-3'
  reverse primer 5'-CTGCTGCTCTGGGTCTCGA-3'
Id4: forward primer 5'-GAACAAGCAGGGTGACAGCA-3'
  reverse primer 5'-CCGCACACCTGGCCAT-3'
Neural-restrictive silencer factor: forward primer 5'-CGGAAGACAAATGCAGGAGTTC-3'
  reverse primer 5'-TTCGGCTTCGTACTGGCAA-3'
Bcl3: forward primer 5'-GCACGGCGCCAACGT-3'
  reverse primer 5'-GCCAGACGCAGAATGCAGA-3'
Krox-20: forward primer 5'-GCGGGAGATGGCATGATC-3'
  reverse primer 5'-GAAGCTACTCGGATACGGGAGA-3'
PTX3: forward primer 5'-TGGACAACGAAATAGACAATGGA-3'
  reverse primer 5'-CTGGCGGCAGTCGCAT-3'
Bradykinin B1 receptor: forward primer 5'-TCAACTGGCCCTTTGGAAGT-3'
  reverse primer 5'-AACAGGTTGGCCTTGATGACC-3'
Guanine nucleotide-binding protein: forward primer 5'-CTGGTAGAACAGCTGAAGATGGAAG-3'
  reverse primer 5'-ACAGTAGGCCATCAAGTCAGCA-3'
ATTP signaling molecule: forward primer 5'-TGCTCCACCCATTTGCTTCT-3'
  reverse primer 5'-TGGCATCCACATGTTTTCCA-3'
Son of sevenless 1: forward primer 5'-CCTGAAAGCCCTCCCTTGTT-3'
  reverse primer 5'-AGATGTAATGGTGAGCTTGAGAAAAC-3'
SHPS-1: forward primer 5'-GGTCAACATCTTCCACACGGTT-3'
  reverse primer 5'-TTTGTGTCCTGGATCTGGGTTA-3'
TRAF1: forward primer 5'-TGTGTGGCCGGACTGTCA-3'
  reverse primer 5'-AGCGCAGGCACAACTTGTAAC-3'
IL-2 receptor gamma: forward primer 5'-TTGGTGCAGTACCGGAGCA-3'
  reverse primer 5'-GCAGGGAGAATCTAGGTTCATGA-3'
Transferrin receptor: forward primer 5'-GACGCTTTGGGTGCTGGT-3'
  reverse primer 5'-GGCAAGTTTCAACAGAAGACCTGT-3'
IL-6: forward primer 5'-TTACACATGTTCTCTGGGAAATCG-3'
  reverse primer 5'-AAGTGCATCATCGTTGTTCATACA-3'
Ki-67 antigen: forward primer 5'-TTTCAGGTCTCTGGAAGCAGTCA-3'
  reverse primer 5'-ATCTCCATAATTGCTTTGATTGCA-3'
Semaphorin F: forward primer 5'-AGTGGTGAGCTCTATGCTGCAA-3'
  reverse primer 5'-CCCAGACTTCGGTAAATGGC-3'
DM-GRASP: forward primer 5'-GCAAAACTGATTGTGGGAATTG-3'
  reverse primer 5'-CAGTAGACGACACCAGCAACG-3'
  1. Shown is a list of forward and reverse primers specific to the murine genes used in quantitative RT-PCR.