Effect of Li+ or PMA on mesothelin and SL-1 expression in C57mg cells. For Li+ treatment, C57mg cells were treated with 10 mM LiCl or 10 mM KCl as control for 6 hours. For PMA treatment, C57mg cells were treated with 1 μM PMA or ethanol as control for 6 hours. RNA was extracted and RT-PCR analysis was performed using primers specific to mesothelin or SL-1 mRNAs. mRNA levels were normalized to HPRT and fold increase shown is relative to KCl treatment in the case of Li+ and to ethanol treatment in the case of PMA. Mean levels were determined from 3 independent experiments.