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Figure 5 | BMC Developmental Biology

Figure 5

From: The Fragilis interferon-inducible gene family of transmembrane proteins is associated with germ cell specification in mice

Figure 5

Expression analysis of the Fragilis family genes in single cells from the region of germ cell specification of E7.5 embryos. (a) shows PCR analysis of cDNAs from three, stella-positive founder PGCs and three surrounding, stella-negative somatic cells. Note that fragilis, fragilis2 and fragilis3 are expressed in PGCs and somatic cells, while fragilis4 and fragilis5 are not detected in any of the cells. (b) shows expression of fragilis, fragilis2 and fragilis3 in single cell cDNAs using Southernblot analysis. GAPDH was used as blotting control. (c) Semi-quantitative expression analysis of the Southernblot data shows that all three Fragilis genes are predominantly expressed in nascent PGCs compared to the somatic cells within the region.

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BMC Developmental Biology

ISSN: 1471-213X

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