A) Confirmation of microarray data using Northern analysis. Northern blot analysis using MSX2, ID2, REST/NRSF, FZD7 (Frizzled7) and Follistatin cDNA probes. NCCIT cells were treated with CCM (-) or Wnt-3A CM (+) for the designated number of hours. 1 ug mRNA was loaded into each lane. GAPDH is shown as a sample loading control. B) MSX1 induction by Wnt-3A CM is dependent on the presence of soluble Wnt-3A protein. C = CCM. Wnt = Wnt-3A CM. Wnt-depleted = Wnt-3A CM. Cells were incubated with the various media for 4 hours.