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Figure 2 | BMC Developmental Biology

Figure 2

From: Hedgehog signal transduction proteins: contacts of the Fused kinase and Ci transcription factor with the Kinesin-related protein Costal2

Figure 2

Direct specific interaction between Cos2 and Ci. (A). GST pull-down assay: In vitro translated, 35S-methionine-labeled Ci or a truncated form of Ci (Ci1-430) before (Input, Lanes 1 and 4) or after incubation with equal amount of, respectively, GST (Lanes 2 and 5) or GST-Cos2 fusion (Lanes 3 and 6). Ci: zinc finger domain in grey, activation domain in black, the approximate cleavage position is around AA703. (B). Yeast two hybrid β-galactosidase assay between various regions of Cos-2 or Rab3 in fusion with the DBD lex-A (Rows I to V) and respectively GGTIIβ, Ci, Ci1-346 or Ci340-445 in fusion with B42 (Columns 1 to 4). See also legends to Figure 1B and 2A.

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BMC Developmental Biology

ISSN: 1471-213X

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