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Figure 8 | BMC Developmental Biology

Figure 8

From: Chitosan/siRNA nanoparticle targeting demonstrates a requirement for single-minded during larval and pupal olfactory system development of the vector mosquito Aedes aegypti

Figure 8

sim knockdown pupal antennal lobe phenotypes. In 36 hr APF wildtype (A1-A3) and control-fed (B1-B3) pupae, ORNs have innervated the antennal lobe and targeted specific glomeruli within the lobe (D7162 dye fills in A1, B1; nc82 staining in A2, B2; overlays are shown in A3 and B3). Distinct glomerular structures are fully formed by this time point (yellow dotted circles in A1, A2, B1, B2). D7162 fills (C1, D1) of pupal ORNs and nc82 staining of the synaptic neuropil (C2, D2; overlays in C3, D3) in Aae sim knockdown pupae (sim430 in C1-C3, sim718 in D1-D3) revealed sparser and more disorganized ORNs and a collapse in glomerular structure in the antennal lobe. ORNs in sim knockdown animals (C1, D1) fail to converge on discrete glomeruli. Dorsal is oriented upward in all panels. Scale bars = 25 microns.

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