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Figure 4 | BMC Developmental Biology

Figure 4

From: Chitosan/siRNA nanoparticle targeting demonstrates a requirement for single-minded during larval and pupal olfactory system development of the vector mosquito Aedes aegypti

Figure 4

Larval antennal lobe defects in sim knockdown animals. In wildtype (A1) and control-fed (B1) L4 larvae, D7162 dye-filled antennal sensory neurons innervate the antennal lobe (highlighted by yellow dots throughout the figure), which is labeled by mAb nc82 (A3, B3). Serotonergic projection neurons are labeled by anti-5HT staining in the antennal lobes of these individuals (A2, B2). An overlay of the three labels is shown in panels A4 and B4 (as well as C4, and D4). sim430(C1-C4) and sim718(D1-D4) animals show a reduction in the number of antennal sensory neurons (D7162 fills in C1, D1) targeting the antennal lobe. The neuropil (nc82 label in C3, D3) and projection neurons (5HT label in C2, D2) are substantially reduced in the antennal lobes of sim knockdown (KD) animals. A subset of antennal sensory neurons which are believed to be gustatory receptors [2] pass through the antennal lobe and project to the subesophageal ganglion (A1, B1; yellow arrowhead). In sim knockdown animals (C1, D1; yellow arrowhead) these neurons are substantially reduced in number. Dorsal is oriented upward in all the panels. Scale bars = 25 microns.

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