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Figure 3 | BMC Developmental Biology

Figure 3

From: Chitosan/siRNA nanoparticle targeting demonstrates a requirement for single-minded during larval and pupal olfactory system development of the vector mosquito Aedes aegypti

Figure 3

sim is required for OR gene expression in the A. aegypti larval antenna. orco and ORs 9, 10, 16, 49, 60, 78, 89, 90, and 100 transcripts are detected in wildtype (A1-J1, respectively) and control-fed (A2-J2, respectively) L4 antennae. Downregulated expression of orco and ORs 9, 10 16, 49, 60, 78, 89, and 100, all of which have adjacent Sim consensus binding sites, is observed in animals that were fed with chitosan/siRNA sim430 (A3-H3, J3 respectively) or sim718 (A4-H4, J4, respectively). orco (A3, A4), OR9 (B3,B4), OR10 (C3, C4), OR49 (E3,E4), and OR89 (H3, H4) expression is not detectable in sim knockdown individuals, while expression of ORs 16 (D3, D4), 60 (F3, F4), 78 (G3, G4), and 100 (J3, J4) is reduced in comparison to wildtype (A1-H1, J1) and control-fed (A2-H2, J2) larvae. Expression of OR90 (I1-I4), which lacks adjacent Sim consensus binding sites, is unchanged in knockdown animals (I3, I4). The proximal ends of antennae are oriented upward in all panels. Scale bars = 25 microns.

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