Figure 4

Ultrastructure of follicular epithelium (A-F) and oocytes at 7th stage of development (G-M) in T. molitor ovary. Follicle cells of control females showing typical pattern (A, B). Low magnification of follicle cell (FC) with well-developed rough endoplasmic reticulum (RER) and Golgi complexes (G) (A). Details of apical part of follicle cell rich in secretory vesicles (SV), mitochondria (M) and rough endoplasmic reticulum (RER) (B). Follicle cells of treated females (C, D). Low magnification of follicle cell (FC) filling with free ribosomes (R) (C). Details of apical part of follicle cell with poorly developed rough endoplasmic reticulum (RER) and Golgi complexes (G) (D). Follicle cells (FC) having numerous vesicles (V) and nucleus (N) with highly condensed chromatin (E). Shrunken follicle cells (FC) forming intercellular spaces (asterisk) (F); inset: autophagic vacuoles (AV) in follicle cell. Oocytes of control females displaying normal pattern (G-I). Cortical ooplasm rich in yolk granules (Y) and lipid droplets (L) (G). Ooplasm with abundance of rough endoplasmic reticulum (RER) and free ribosomes (R) (H). Annulate lamellae (AL) in ooplasm (I). Oocytes of treated females (J-M). Cortical ooplasm with small yolk granules (Y) and lipid droplets (L) (J). Rough endoplasmic reticulum (RER) and ribosomes (R) in ooplasm (K). Cortical ooplasm with autophagic vacuoles (AV) (L). Core of the oocyte containing autophagic vacuoles (AV) (M). M – mitochondria; Mv – microvilli; N - nucleus; Nu – nucleolus; O – oocyte; RER - rough endoplasmic reticulum; V – vacuoles; VM – vitelline membrane. Scale bars: (A, C, G, J, M) 2 μm; (B, E, F, inset, I, L) 1 μm; (D, H, K) 0.5 μm. Females were injected with saline (control) or 10 nmole Neb-colloostatin (treated) on day 3 and assayed on day 4.