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Figure 2 | BMC Developmental Biology

Figure 2

From: The natural insect peptide Neb-colloostatin induces ovarian atresia and apoptosis in the mealworm Tenebrio molitor

Figure 2

Confocal micrographs of control (A, C, E) and Neb -colloostatin-treated (B, D, E) terminal follicles of T. molitor . In control follicular cells F-actin stained with Oregon Green phalloidin is localized subcortically and maximum patency is visible (arrow) (A). Follicular cells of treated females show no Oregon Green phalloidin stain indicating depolimerization of F-actin (B). In control follicular cells stained with SR-VAD-FMK active caspase is not detected (C). In follicular cells of treated females SR-VAD-FMK stain shows caspase activation (D). In control follicular cells stained with propidium iodide, nuclei have normal pattern of chromatin (E). In follicular cells of treated females stained with propidium iodide, nuclei are shrunk, chromatin organization is changed and DNA fragmentation is visible (arrow) (F). Females were injected with saline (control) or 10 nmole Neb-colloostatin (treated) on day 3 and assayed on day 4.

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