Figure 3

Validation of LC/MS/MS. Immunofluorescent antibody staining and mean densitometric sum for β1 integrin, vimentin, and dystroglycan, comparing control, 5 dpa and 12 dpa sections of regenerating froglet hindlimbs. A-D, β1 integrin; E-H, vimentin; I-L, dystroglycan. The 1, 5, 7 and 12 dpa fold changes for each of these proteins were: β1 integrin-1.05, 1.28, 2.18, 2.80; vimentin—1.07, 1.94, 2.30, 3.15; dystroglycan-1.20, -1.40, -1.49, -2.02. The immunofluorescence and densitometry data thus agree well with the LC/MS/MS proteomic data.