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Figure 4 | BMC Developmental Biology

Figure 4

From: Histone deacetylase expression patterns in developing murine optic nerve

Figure 4

NKX2.2 and SOX2 localization in developing murine optic nerve. Optic nerve sections at E16 (A-C), P5 (D-F), P30 proximal to the eye (G-I) and P30 distal to the eye (J-L), were triple-labeled with DAPI (blue), SOX2 (green) and NKX2.2 (red). An overlay of NKX2.2 and DAPI is shown to aid in orientation of the images (A, D, G, J). At E16 and P5, NKX2.2+ cells were not detected in the proximal or distal optic nerve (B, E). At P30, NKX2.2+ cells were not detected in the proximal nerve (H), but were found in the distal optic nerve (K). Composite images in C, F, I and L show z stacks of labeled optic nerve; horizontal yellow lines correspond the x axis plane and vertical yellow lines corresponds to the y axis. The x, z axis is shown at the bottom of each panel, while the y, z axis is shown to the right of the panel. Graph shown (M), represents the percentage of cells positively labeled for SOX2 alone, NKX2.2 alone or both SOX2 and NKX2.2 together. Bars show mean ± SEM of three samples per timepoint. Abbreviations: R; retina, ON; optic nerve. Scale bars = 50 μm. Scale bar in C applies to A-L.

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