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Figure 3 | BMC Developmental Biology

Figure 3

From: Histone deacetylase expression patterns in developing murine optic nerve

Figure 3

PAX2 and SOX2 localization pattern in developing murine optic nerve . Optic nerve sections at E16 (A-C), P5 (D-F) and P30 (G-I) were triple-labeled with DAPI (blue), PAX2 (green) and SOX2 (red). An overlay of PAX2 and DAPI (A, D, G) is shown to aid in orientation of the images. Immunolabel of SOX2 and PAX2 (arrows) showed a majority of cells to be colabeled at all stages examined. Composite images in C, F and I show z stacks of optic nerve; horizontal yellow lines correspond the x axis plane and vertical yellow lines corresponds to the y axis. The x, z axis is shown at the bottom of each panel, while the y, z axis is shown to the right of the panel. Graph shown (J), represents the percentage of cells positively labeled for SOX2 alone, PAX2 alone or SOX2 and PAX2 together in the proximal optic nerve. Bars show mean ± SEM of three samples per timepoint. Abbreviations: R; retina, ON; optic nerve. Scale bars = 50 μm. Scale bar in C applies to A-I.

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