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Table 3 Effect of Cyclopamine on proliferation of regenerating cells

From: Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole

Tissues Notochord Spinal cord Mesenchyme
Chemicals ET TO CP ET TO CP ET TO CP
% BrdU+ cells 52.3 54.9 59.3 61.2 57.5 35.6 66.9 70.7 36.2
SD 22.5 18.2 13.8 16.7 13.6 11.6 14.5 10.5 9.6
number of sections 18 20 21 18 16 15 14 27 36
p-value (ET) - 0.65 0.19 - 0.24 1.0 E-5 - 0.83 8.4 E-12
p-value (TO) - - 0.81 - - 2.1 E-5 - - 2.3 E-20
  1. Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM) or cyclopamine (CP, 2.5 μM). Four tadpoles for each condition were fixed at day 2.5 after 12 h BrdU labeling. Mean rate of proliferating cells were determined on sagittal (notochord and spinal cord) or frontal (mesenchymal region) sections. Student’s t-test was used to identify differences between indicated groups.