Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole

Table 3 Effect of Cyclopamine on proliferation of regenerating cells

Tissues	Notochord			Spinal cord			Mesenchyme
Chemicals	ET	TO	CP	ET	TO	CP	ET	TO	CP
% BrdU⁺ cells	52.3	54.9	59.3	61.2	57.5	35.6	66.9	70.7	36.2
SD	22.5	18.2	13.8	16.7	13.6	11.6	14.5	10.5	9.6
number of sections	18	20	21	18	16	15	14	27	36
p-value (ET)	-	0.65	0.19	-	0.24	1.0 E-5	-	0.83	8.4 E-12
p-value (TO)	-	-	0.81	-	-	2.1 E-5	-	-	2.3 E-20

Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM) or cyclopamine (CP, 2.5 μM). Four tadpoles for each condition were fixed at day 2.5 after 12 h BrdU labeling. Mean rate of proliferating cells were determined on sagittal (notochord and spinal cord) or frontal (mesenchymal region) sections. Student’s t-test was used to identify differences between indicated groups.

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