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Table 2 Effect of cyclopamine on length of regenerating notochord

From: Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole

 

Day 3

Day 5

Chemicals

ET

TO

CP

CP + PM

ET

TO

CP

CP + PM

Number

8

8

8

8

8

8

7

8

Mean (μm)

301.5

274.5

198.5

254.9

811.3

590.7

263.3

591.9

SD

81.8

78.4

28.6

59.9

147.5

251.4

56.4

146.4

P-value (ET)

-

0.26

2.3 E-3

0.12

-

2.5 E-2

2.3 E-7

4.9 E-3

P-value (TO)

-

-

1.1 E-2

0.3

-

-

2.6 E-3

0.5

P-value (CP)

-

-

-

0.98

-

-

-

4.6 E-5

  1. Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM), cyclopamine (CP, 2.5 μM), or cyclopamine plus purmorphamine (PM, 0.25 μM). Mean length of the regenerated notochord was determined at day 3 and day 5. Student’s t-test was used to identify differences between indicated groups.