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Table 2 Effect of cyclopamine on length of regenerating notochord

From: Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole

  Day 3 Day 5
Chemicals ET TO CP CP + PM ET TO CP CP + PM
Number 8 8 8 8 8 8 7 8
Mean (μm) 301.5 274.5 198.5 254.9 811.3 590.7 263.3 591.9
SD 81.8 78.4 28.6 59.9 147.5 251.4 56.4 146.4
P-value (ET) - 0.26 2.3 E-3 0.12 - 2.5 E-2 2.3 E-7 4.9 E-3
P-value (TO) - - 1.1 E-2 0.3 - - 2.6 E-3 0.5
P-value (CP) - - - 0.98 - - - 4.6 E-5
  1. Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM), cyclopamine (CP, 2.5 μM), or cyclopamine plus purmorphamine (PM, 0.25 μM). Mean length of the regenerated notochord was determined at day 3 and day 5. Student’s t-test was used to identify differences between indicated groups.