Figure 2

Effect of cyclopamine on the tail regeneration. The tail-amputated tadpole was maintained in the presence of the indicated compound. ET, 0.1% ethanol. TO, 2.5 μM tomatidine. CP, 2.5 μM cyclopamine. CP + PM, 2.5 μM cyclopamine and 0.25 μM purmorphamine. (A-D) Regenerated tails were observed under a stereoscopic microscope at day 7. Bar, 500 μm. (E-H) Histological analysis of the regenerated tail at day 7. Sagittal sections were stained with hematoxylin and eosin. nc, notochord. Bar, 100 μm. A pair of arrowheads marks the amputation plane. (I, J) The length of the regenerated tail and notochord at day 5. Mean length of the regenerated tail (I) or the notochord (J) was indicated with the standard deviation. Detail is shown in Tables 1 and 2. *, p-value < 0.05. **, p-value <0.01. (K) Gene expression analysis with RT-PCR. RNA from the control or cyclopamine-treated tails was analyzed at day 2.5 or day 5.